Blooming Processes in Flowers Studied By Dynamic Electronic Speckle Pattern Interferometry (DESPI)

A dynamic electronic speckle pattern interferometry (ESPI) method was applied to study the blooming process in two kinds of flowers, the lily and the tulip. The complicated displacement fields of the flower petals were precisely monitored throughout the entire blooming process. The optical setup is a Fizeau type interferometer with relatively high robustness to air turbulence and vibrations. For quantitative evaluation of the displacement field, the subtraction-addition method (SAM), which utilizes only the digital signal processing of a sequence of images, was implemented. Measurements with accuracy in the range of λ/10 were achieved.     

吉林省长白山野生百花中微量元素的分析

应用电感耦合等离子发射光谱仪（ICP-AES），首次对采自长白山野生百合花进行了微量元素成分分析，其结果为综合开发利用长白山野生百合花资源提供科学依据。     

Determination and Comparison of Phytoestrogens in Both Crude and Parched Flos Sophorae Immaturus by Capillary Electrophoresis with Electrochemical Detection

A method using high-performance capillary electrophoresis with electrochemical detection (CE-ED) has been developed for content comparison of phytoestrogens in both crude and parched Flos sophorae immaturus. Genistin, genistein, rutin, kaempferol and quercetin are important active constituents in this plant. The effects of several factors, such as acidity and concentration of running buffer, separation voltage, applied potential and injection time, were investigated to find the optimum conditions. Detection limits (S/N=3) ranged from 1.1 × 10^–7 to 2.8 × 10^–7gmL^–1 for all five analytes. This method was successfully used to analyse both crude and parched Flos sophorae immaturus after a relatively simple extraction procedure, and the assay results were satisfactory.     

Two New Antibacterial Flavanones from Sophoraflavescens

The dry root of Sophora flavescens, which is a well-known Chinese herbal medicine, has been used for the treatment of diarrhea, gastrointestinal hemorrhage and eczema1. Here we report the structure elucidation of two new flavanones isolated from this plan…     

蚕豆花精油化学成份的研究

用毛细管气相色谱-质谱-数据系统联用技术分析了蚕豆花精油的化学成份。已鉴定的十六个化学成份为芳樟醇、松油醇、4-甲氧苯基乙酮及其异构体、丁香酚及其异构体、2,6-二甲氧基-4-丙基-2-苯酚、十四碳烷酸、6,10,14-三甲基十五烷酮-2、十六碳烷酸、十七碳烷酸、十八碳三烯醛、十八碳二烯酸的异构体和十八碳烷酸。它们共占精油含量的90%左右。     

毛细管区带电泳法研究不同炮制方法对槐花、槐米中芦丁和槲皮素含量的影响

建立了一种毛细管区带电泳(CZE)法测定芦丁和槲皮素的方法,利用该方法研究了不同炮制方法对槐花、槐米中芦丁和槲皮素含量的影响。研究了缓冲溶液pH和浓度、分离电压、有机添加剂的浓度和进样时间对分离的影响,在优化条件下,上述两种物质在5 min内实现良好分离。芦丁和槲皮素峰面积和质量浓度分别在0.04~1.8和0.02~2.0 g.L-1浓度范围内呈线性关系,线性相关系数分别为0.999 2和0.999 4,检出限分别为8和5 mg.L-1。试验结果表明,炮制方法影响槐花和槐米中芦丁、槲皮素的含量。     

Simultaneous determination of phytoestrogens in different medicinal parts of Sophora japonica L. by capillary electrophoresis with electrochemical detection

A high-performance capillary electrophoresis with electrochemical detection (CE-ED) method has been developed for the determination of phytoestrogens from the pericarps and seeds of Sophora japonica L. in this work. Genistin, genistein, rutin, kaempferol and quercetin are important bioactive constituents in these plants. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on the CE-ED procedure were investigated. Under the optimum conditions, the five analytes could be well separated within 18 min in a 75 cm length capillary (i.d. 25 microm) at the separation voltage of 16 kV in a 50 mmol L(-1) borax running buffer (pH 9.0). A 300 microm diameter carbon disk electrode was used as the working electrode positioned carefully opposite the outlet of the capillary in a wall-jet configuration at the potential of +950 mV (vs SCE). Detection limits (S/N = 3) ranged from 1.1 x 10(-7) to 2.8 x 10(-7) g mL(-1) for all fi ve analytes. This method was successfully used to analyse dried Flos sophorae immaturus, pericarps and seeds of dried Fructus sophorae after a relatively simple extraction procedure, and the assay results were satisfactory.     

On the weighted Kneser-Poulsen conjecture

Suppose that p = (p1, p2, …, pN) and q = (q1, q2, …, qN) are two configurations in , which are centers of balls B ^d (p _i , r _i ) and B ^d (q _i , r _i ) of radius r _i , for i = 1, …, N. In [9] it was conjectured that if the pairwise distances between ball centers p are contracted in going to the centers q, then the volume of the union of the balls does not increase. For d = 2 this was proved in [1], and for the case when the centers are contracted continuously for all d in [2]. One extension of the Kneser-Poulsen conjecture, suggested in [6], was to consider various Boolean expressions in the unions and intersections of the balls, called flowers, where appropriate pairs of centers are only permitted to increase, and others are only permitted to decrease. Again under these distance constraints, the volume of the flower was conjectured to change in a monotone way. Here we show that these generalized Kneser-Poulsen flower conjectures are equivalent to an inequality between certain integrals of functions (called flower weight functions) over , where the functions in question are constructed from maximum and minimum operations applied to functions each being radially symmetric monotone decreasing and integrable. Research supported in part by NSF Grant No. DMS-0209595.     

The colonic metabolism differences of main alkaloids in normal and colitis mice treated with Coptis chinensis Franch. and Sophora flavescens Ait. herbal pair using liquid chromatography-high resolution mass spectrometry method combined with chemometrics

Coptis chinensis Franch. and Sophora flavescens Ait. is a herbal pair frequently used in treating ulcerative colitis. However, the bio-disposition profile of the major components in the inflamed gut remains unclear, which is essential to understand the pharmacological material basis of this herb pair. Here we established an integral quantitative and chemometric method to deduce the colonic metabolism differences of this herbal pair in normal and colitis mice. With this LC-MS method, a total of 41 components have been found in the Coptis chinensis Franch. and Sophora flavescens Ait. extract, and 28 metabolites were found in the colon after oral administration. Alkaloid and its phase I metabolites were the main components in the colon of normal and colitis mice. The results of principal component analysis at 6 h after oral administration showed significant colonic metabolism differences between normal and colitis mice. Heamap results showed that colitis induced significant changes in the colonic bio-disposition of this herbal pair extract. In particular, in the context of colitis, the phase I metabolism of berberine, coptisine, jatrorrhizine, palmatine,and epiberberine has been inhibited. These results may provide a basis for understanding the pharmacological material basis of Coptis chinensis Franch. and Sophora flavescens Ait. in treating ulcerative colitis.     

洋槐叶和槐花中营养成分分析的研究

用现代仪器分析方法对洋槐花和槐叶中蛋白质、还原糖、总糖、抗坏血酸、氨基态氮含量及微量元素含量进行了测试,并对其作为饲料的价值进行了分析。结果显示,洋槐花和槐叶作为饲料资源具有良好的应用前景。